The lab says fixation will bring about the dyes to leak through the cells. I do think the assumption is that fixation may well hurt cell membranes. I did locate a paper which describes fixation applying methanol or acetic acid, right after staining that has a DCFDA derivative. DCFDA will https://2-fdckhcl56542.aioblogs.com/82935279/the-2-minute-rule-for-2-fdck-hcl